DANIEL BRANTON Higgins Research Professor of Biology Email: dbranton@harvard.edu Phone: (617) 495-2646 Mail: 16 Divinity Ave, Room 2000A, Cambridge MA, 02138 Branton Lab Homepage Nanopore Group Homepage Current Members of the Branton Lab Past Members of the Branton Lab List of Publications from PubMed

Research:

Nanopore Technology:

A novel technology for probing, and eventually sequencing, individual DNA molecules using single-channel recording techniques has been conceived. Single molecules of DNA are drawn through a small channel or nanopore that functions as a sensitive detector. The detection schemes being developed will transduce the different chemical and physical properties of each base into a characteristic electronic signal. Nanopore sequencing has the potential of reading very long stretches of DNA at rates exceeding 1 base per millisecond.

For more information about this project, please go to http://mcb.harvard.edu/branton/index.htm.

Selected Publications:

Fologea, D., E. Brandin, J. Uplinger, D. Branton, and J. Li. 2007. DNA conformation and base number simultaneously determined in a nanopore. Electrophoresis 28: 3186-3192.

Kim, Y.-R., P. Chen, M.J. Aziz, D. Branton, and J.J. Vlassak. 2006. Focused ion beam induced deflections of freestanding thin films. J. Appl. Phys. 100: 104322-1 - 104322-9.

Park, S.Y., C.J. Russo, D. Branton, and H.A. Stone. 2006. Eddies in a bottleneck: An arbitrary Debye length theory for capillary electroosmosis. J. Colloid Interface Sci. 297: 832-839.

King, G.M., Schurmann, G., Branton, D. & Golovchenko, J.A. 2005. Nanometer patterning with ice. Nano Letters 5, 1157-1160.

Chen, P., J. Gu, E. Brandin, Y.R. Kim, Q. Wang, and D. Branton. 2004. Probing single DNA molecule transport using fabricated nanopores.  Nano Letters 4: 2293-2298.
 
Wang, H., J.E. Dunning, A. P.-H. Huang, J.A. Nyamwanda, and D. Branton. 2004. DNA heterogeneity and phosphorylation unveiled by single-molecule electrophoresis.  Proc. Natl. Acad. Sci. U.S.A. 101: 13472-13477.

Chen, P., T. Mitsui, D.B. Farmer, J. Golovchenko, R.G. Gordon, and D. Branton. 2004. Atomic layer deposition to fine-tune the surface properties and diameters of fabricated nanopores. Nano Letters 4: 1333-1337.

Sauer-Budge, A.F., J.A. Nyamwanda, D.K. Lubensky, and D. Branton. 2003. Unzipping kinetics of double-stranded DNA in a nanopore. Phys. Rev. Lett. 90:23801-1 - 23801-4.

Deamer, D. and D. Branton. 2002. Characterization of nucleic acids by nanopore analysis. Acc. Chem. Res. 35:817-825.

Meller, A. and D. Branton. 2002. Single molecule measurements of DNA transport through a nanopore. Electrophoresis 23: 2583-2591.

Branton, D. and A. Meller. 2002. Using nanopores to discriminate between single molecules of DNA. In Structure and Dynamics of Confined Polymers (Kasianowicz, J.J., Kellermayer, M.S.Z., and Deamer, D.W., eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 177-185.

Wang, H., and D. Branton. 2001. Nanopores with a spark for single-molecule detection. Nature Biotechnology. 19:622-623.

Li, J., D. Stein, C. McMullan, D. Branton, M.J. Aziz, and J.A. Golovchenko. 2001. Ion-beam sculpting at nanometre length scales. Nature. 412:166-169.

Meller, A., L. Nivon, and D. Branton. 2001. Voltage-driven DNA translocations through a nanopore. Phys. Rev. Lett. 86:3435-3438.

Meller, A., L. Nivon, E. Brandin, J. Golovchenko, and D. Branton. 2000. Rapid nanopore discrimination between single polynucleotide molecules. Proc. Natl. Acad. Sci. USA 97: 1079-1084.