Harvard University - Department of Molecular & Cellular Biology


Higgins Professor of Biochemistry

Email: guidotti@fas.harvard.edu
Phone: 617-495-2308

Mail: NW 140.49
Northwest Building
52 Oxford St
Cambridge, MA  02138

History of Biochemistry at Harvard
Members of the Guidotti Lab
List of Publications from PubMed


MCB 176. Biochemistry of Membranes
Catalog Number: 3186  View Course Website
Term: Fall Term 2014-2015.
Instructor: Guido Guidotti
Course Level: For Undergraduates and Graduates
Description: A course on the properties of biological membranes, essential elements for cell individuality, communication between cells, and energy transduction. Topics include: membrane structure; membrane protein synthesis, insertion in the bilayer and targeting; transporters, pumps and channels; electron transport, H+ gradients and ATP synthesis; membrane receptors, G proteins and signal transduction; membrane fusion.
Prerequisite(s): MCB 60 or MCB 52 and MCB 54 are recommended but not required.
Meetings: M., W., 2–4, M., at 4
MCB 178. Biochemistry of Protein Complexes
Catalog Number: 63269  View Course Website
Term: Spring Term 2014-2015.
Instructor: Guido Guidotti
Course Level: For Undergraduates and Graduates
Description: A course on the properties of protein complexes that serve as supports, machines and motors. Topics include : hemoglobin, actin filaments, myosin function, microtubules, kinesin, metabolon, photosynthesis, rotary motors, nuclear pores and transport, proteasome. Lecture on Monday is followed by student presentations on Wednesday. The course involves reading two research papers per week, and writing a research proposal.
Prerequisite(s): Chemistry 27 and MCB 60 or MCB 54 are recommended, but not required.
Meetings: M., W., 2:30-4
MCB 391. Biochemistry
Catalog Number: 4888  View Course Website
Term: Fall Term And Spring Term 2014-2015.
Instructor: Guido Guidotti
Course Level: Exclusively for Graduates
FRSEMR 26z. What is Life?
Catalog Number: 20722  View Course Website
Term: Fall Term 2014-2015.
Instructor: Guido Guidotti
Course Level: Primarily for Undergraduates
Description: This seminar considers the conditions of a cell necessary to support life. The proposal is to find a definition for a living system using information and principles of biology, chemistry and physics to characterize some central properties of living cells, like energy and material uptake and use, cellular crowding, diffusion and molecular interactions, homeostasis and growth.
Note: Open to Freshmen only.
Meetings: Th., 2:30-4
(View all MCB Courses)


Structure and Function of Membrane Proteins

The function and regulation of membrane proteins involved in the transfer of solutes (channels and transporters) and of information (receptors) across the plasma membrane are the subjects of research in this laboratory.

The particular proteins under study are the insulin receptor, the (Na,K) ATPase, an insulin-activated cation channel, channels and transporters for ATP, and an ecto-apyrase.


Boyum, R., and Guidotti, G. Glucose dependent, cAMP mediated ATP efflux from Saccharomyces cerevisiae. Microbiology 143, 1901-1908 (1997).

Booth, J. and Guidotti, G. Phosphate transport in yeast vacuoles. J. Biol. Chem. 272, 20408-20413 (1997).

Coppi, M.V., Compton, L.A., and Guidotti, G. Isoform specific effects of charged residues at the borders of the M1-M2 loop of the Na,K-ATPase a subunit. Biochemistry 38, 2494-2505 (1999).

Grinthal, A., and Guidotti, G. Substitution of His59 converts CD39 apyrase into an ADPase in a quaternary structure dependent manner. Biochemistry 39, 9-16 (2000).

McGeoch, J.E., McGeoch, M.W., Mao, R., and Guidotti, G. Opposing actions of cGMP and calcium on the conductance of the F(0) subunit c pore. Biochem. Biophys. Res. Commun. 274, 35-840 (2000).

Zhong, X., Malhotra, R., and Guidotti, G. Regulation of yeast ectoapyrase Ynd1p activity by activator subunit Vma13p of vacuolar H-ATPase. J. Biol. Chem. 275, 35592- 35599 (2000).

Lee, K., Jung, J., Kim, M., and Guidotti, G. Interaction of the alpha subunit of Na, K- ATPase with cofilin. Biochem. J. 353, 377-385 (2001).

Chen, W., and Guidotti, G. Soluble apyrases release ADP during ATP hydrolysis. Biochem. Biophys. Res. Commun. 282, 90-95 (2001).

Zhong, X., Malhotra, R., Woodruff, R., and Guidotti, G. Mammalian Plasma Membrane Ecto-NTPDase1, CD39, Is Not Active Intracellularly: The N-Glycosylation State of CD39 Correlates with Surface Activity and Localization. J. Biol. Chem. 276, 41518- 41525 (2001).

Chen, W. and Guidotti, G. The Metal Coordination of sCD39 during ATP Hydrolysis. BMC Biochemistry 2, 9 (2001).

Grinthal, A., and Guidotti, G. Transmembrane Domains Confer Different Substrate Specificities and ADP Hydrolysis Mechanisms on CD39, CD39L1, and Chimeras. Biochemistry 41, 1947-1956 (2002).

Wu, J.J., and Guidotti, G. Construction and Characterization of a Monomeric Insulin Receptor. J. Biol. Chem. 277,  27809-27817 (2002)

Zhong, X., Malhotra, R., and Guidotti, G. ATP transport into the Golgi and extracellular  release require Mcd4 protein and the vacuolar H+-ATPase.  J. Biol. Chem. 278, 33436-33444 (2003).

updated: 12/06/2016