USING LINEAR MICRO-COLONIES TO FOLLOW PROMOTER ACTIVITY ACROSS GENERATIONS OF E.COLI
Source: Cluzel Lab
Genealogy in a micro-linear colony of the activity of the MAR (multi-antibiotic resistance) promoter in individual E. coli in response to a steady exposure of salicylate (1mM). The Venus yellow fluorescent protein reports the activity of this promoter as described in the original proposal. The concentration of Venus is calibrated using Fluorescent Correlation Spectroscopy and is color-coded from dark-green to white, which corresponds respectively to low and high level of expression of the reporter. Here we can visualize to what extent the activity of the MAR promoter, which governs resistance to antibiotics, is inherited from generation to generation.