Harvard University - Department of Molecular & Cellular Biology

CRAIG P HUNTER

Hunter
Professor of Molecular and Cellular Biology

Email: hunter@mcb.harvard.edu
Phone: 617-495-8309

Mail: BL 3044
The Biological Labs
16 Divinity Ave
Cambridge, MA  02138

Hunter Lab Homepage
Members of the Hunter Lab
List of Publications from PubMed

Courses

MCB 101. Human Genetics
Catalog Number: 14764  View Course Website
Term: Fall Term 2014-2015.
Instructor: Craig Hunter
Course Level: For Undergraduates and Graduates
Description: Genomic information is accelerating the discovery and characterization of the molecular and cellular basis of human health and disease. This new lecture/discussion course will explore how knowledge from new technologies is used to advance our understanding of human biology. Topics will include personal genomics, understanding genome-wide associated studies, epigenetics, gene-environment interactions, and complex traits, the importance of model organisms to investigate molecular mechanisms, and the prospects for cancer genomics and gene/genome therapy. This lecture/discussion course will rely extensively on primary literature and contemporary review articles. Students will actively participate in class discussions and prepare four written summaries of assigned articles and two literature-based research projects (one in the middle of the term and one at the end of the term) that critically assess the scientific basis of popular news articles and consumer-targeted genomics information.
Prerequisite(s): LS 1a and LS 1b, MCB 60 or MCB52 and MCB54.
Meetings: M., W., 10-11:30
MCB 157. Developmental Genetics and Genomics
Catalog Number: 20809  View Course Website
Term: [Fall Term .]
Instructor: Craig Hunter
Course Level: For Undergraduates and Graduates
Description: Our goal is for students to gain a fundamental understanding of the genetic control of development in four genetically accessible animal models; the nematode C. elegans, the fruit fly Drosophila melanogaster, the zebra fish Danio rio, and the mouse Mus musculus. A focus of the course is to compare and contrast genetic analysis of and the genetic control of developmental processes and mechanisms in these four organisms. The course consists of lectures, student presentations, and written assignments.
Prerequisite(s): LS 1b
Meetings: M., W., 12-1:30
MCB 322. Genetics and Development
Catalog Number: 7290  View Course Website
Term: Fall Term And Spring Term 2014-2015.
Instructor: Craig Hunter
Course Level: Exclusively for Graduates
(View all MCB Courses)

Research

Systemic RNAi and intercellular RNA transport

We are interested in the mechanisms of intercellular RNA transport that supports systemic RNAi in C. elegans and are investigating the developmental and signaling defects associated with mutations in these RNA transport proteins in C. elegans and mice.

A remarkable property of RNA interference (RNAi) in C. elegans is its association with intercellular RNA transport pathways. This linkage mobilizes dsRNA-silencing signals and enables silencing to spread from the site of initiation throughout the animal and to the progeny. This phenomenon, known as systemic RNAi, is a conserved process among many multicellular organisms. Through genetic analysis, we have isolated systemic RNAi defective mutants (sid) and have identified the corresponding proteins (SID). SID-1 is a widely conserved dsRNA channel that selectively and specifically transports dsRNA into cells and is essential for systemic RNAi. A mammalian SID-1 homolog has been implicated in cytoplasmic delivery of modified siRNAs, suggesting that dsRNA transport is a conserved function for this family of channel proteins. SID-2 is a putative dsRNA receptor that is expressed and localized exclusively to the luminal membrane of the intestine. SID-2 transports ingested dsRNA across the intestinal epithelium into the animal to trigger RNAi. This process of sequence-specific gene silencing in response to environmentally-encountered dsRNA, known as environmental RNAi, is widespread throughout nature, including in mammals. The long-term objective of our research is to understand the physiological importance and mechanism of intercellular RNA transport in animals.

Publications

Jose AM, Smith JJ, Hunter CP. Export of RNA silencing from C. elegans tissues does not require the RNA channel SID-1. Proc Natl Acad Sci U S A. 2009 106(7):2283-8.

Shih JD, Fitzgerald MC, Sutherlin M, Hunter CP. The SID-1 double-stranded RNA transporter is not selective for dsRNA length. RNA. 2009 15(3):384-90.

Whangbo JS, Hunter CP. Environmental RNA interference. Trends Genet. 2008 24(6):297-305.

Jose AM, Hunter CP. Transport of sequence-specific RNA interference information between cells. Annu Rev Genet. 2007 41:305-30.

Winston WM, Sutherlin M, Wright AJ, Feinberg EH, Hunter CP. Caenorhabditis elegans SID-2 is required for environmental RNA interference. Proc Natl Acad Sci U S A. 2007 104(25):10565-70.

Schott DH, Cureton DK, Whelan SP, Hunter CP. An antiviral role for the RNA interference machinery in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2005 102(51):18420-4.

Feinberg EH, Hunter CP. Transport of dsRNA into cells by the transmembrane protein SID-1. Science. 2003 301(5639):1545-7.

Winston WM, Molodowitch C, Hunter CP. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1. Science. 2002 295(5564):2456-9.

Hunter CP. Gene silencing: shrinking the black box of RNAi. Curr Biol. 2000 10(4):R137-40.

Hunter CP. Genetics: a touch of elegance with RNAi. Curr Biol. 1999 9(12):R440-2.

updated: 06/30/2015