The Kahne Lab is interested in understanding the biogenesis of the cell envelope of Gram-negative bacteria, in particular peptidoglycan biosynthesis and outer membrane assembly. The assembly of this organellar membrane must be accomplished outside the cell in the absence of an obvious energy source. My research focuses on identifying and understanding the machinery necessary for proper assembly of this membrane barrier, as well as the mechanisms that lead to defects. Because the outer membrane creates an effective permeability barrier to most antibiotics, understanding how to interfere with its assembly could provide new targets for antibiotic discovery.
S. Okuda, E. Freinkman, D. Kahne. Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in E. coli. Science 2012; 338:1214-7 PDF
S.S. Chng, M. Xue, R.A. Garner, H. Kadokura, D. Boyd, J. Beckwith, D. Kahne. Disulfide rearrangement triggered by translocon assembly controls lipopolysaccharide export. Science 2012; 337:1665-8 PDF
C.L. Hagan, S. Kim, D. Kahne. Reconstitution of outer membrane protein assembly from purified components. Science 2010; 328:890-2 PDF
S. Kim, J.C. Malinverni, P. Sliz, T.J. Silhavy, S.C. Harrison, D. Kahne. Structure and function of an essential component of the outer membrane protein assembly machine Science 2007; 317:961-964 PDF
T. Wu, J. Malinverni, N. Ruiz, S. Kim, T.J. Silhavy, D. Kahne Identification of a multi-component complex required for outer membrane biogenesis in Escerichia coli. Cell 2005; 121:235-246